Serostatus of IgG Antibody Against Mumps Virus in Healthcare Workers at a Tertiary Care Hospital, Nalgonda

Background: Mumps remains an important vaccine-preventable viral infection because outbreaks continue to occur in adult and occupational groups despite childhood vaccination. Healthcare workers require adequate immunity to prevent occupational acquisition and onward transmission within hospitals. The objective is to determine the serostatus of mumps-specific immunoglobulin G antibodies among healthcare workers in a tertiary care hospital at Nalgonda and to assess its association with measles-mumps-rubella vaccination dose and gender. Material and Methods: This prospective cross-sectional study was conducted over six months at Government General Hospital, Nalgonda. Healthcare workers with documented receipt of one or two doses of measles-mumps-rubella vaccine were enrolled. Participants with previous mumps infection, immunocompromising conditions, or immunosuppressive therapy were excluded. Venous blood samples were collected, serum was separated, and mumps-specific immunoglobulin G was detected using the Bio-Rad Mumps IgG enzyme-linked immunosorbent assay. Equivocal results were considered non-protective for analysis. Associations were assessed using the chi-square test. Results: A total of 194 healthcare workers were included; 118 (60.8%) were female and 76 (39.2%) were male. Overall, 126 participants were seropositive, giving a mumps IgG seropositivity rate of 65.0%, while 68 (35.0%) were seronegative. Seropositivity was significantly higher among double-dose recipients than single-dose recipients (82.1% versus 51.8%; chi-square = 19.24, p < 0.001). Double-dose recipients had 1.59 times higher seropositivity than single-dose recipients. Gender-wise seropositivity was comparable between females and males (65.2% versus 64.5%; p = 0.911). Conclusion: Mumps IgG seropositivity among healthcare workers was suboptimal, with one-third remaining susceptible. Completion of the two-dose measles-mumps-rubella schedule was strongly associated with detectable immunity, supporting institutional screening and targeted booster vaccination. Keywords: Mumps virus; Healthcare workers; Mumps IgG; MMR vaccine; Seroprevalence; Occupational health; ELISA.